中文摘要：

目的生物信息学预测并实验验证mmu-miR-107的靶基因。方法利用Target Scan、miRanda、Clip-seq及miRDB预测mmu-miR-107靶基因,分别构建含有候选靶基因（Cacna2d1、Capza2、Celsr2、Csnk1g2和Tmem47）3＇UTR的荧光素酶报告载体p GL3,通过双荧光素酶检测系统、时实定量PCR进一步验证预测的靶基因。结果成功构建分别含有5个候选靶基因3＇UTR的荧光素酶报告载体;与mi R-NC组相比,mi R-107组共转染p GL3-Cacna2d1 3＇UTR、p GL3-Cav13＇UTR的荧光素酶活性均显著下降（P〈0.01或0.05）;其余候选靶基因Capza2、Celsr2、Csnk1g2和Tmem47的共转染组荧光素酶活性差异无统计学意义（P〉0.05）;过表达或下调C2C12细胞的miR-107,可有效降低或提高Cacna2d1的mRNA水平表达（P〈0.05）。结论初步验证Cacna2d1是mmu-miR-107的靶基因。

英文摘要：

Objective To predict and confirm the target genes of mmu-mi R-107. Methods Target genes of mmu-mi R-107 was predicted by Target Scan, mi Randa, Clip-seq and mi RDB, the 3＇UTR of candidate target genes（Cacna2d1, Capza2, Celsr2, Csnk1g2 and Tmem47） were inserted in to the plasmid p GL3. Candidate target genes were verified by luciferase reporter assay, q-PCR. Results The luciferase reporter plasmid of the five candidate target genes were successfully constructed. Compared with mi R-NC Group, the luciferase activity of C2C12 cells co-transfected with p GL3-Cacna2d1 3＇UTR and p GL3-Cav1 3＇UTR in mi R-107 Group was decreased by 33.4%（P〈0.01 or 0.05）; there was no statistical difference in luciferase activity among the C2C12 cells co-transfected with other candidate target genes including Capza2, Celsr2, Csnk1g2 and Tmem47（P〉0.05）; Overexpressing or down-regulating mi R-107 in C2C12 cells, can effectively reduce or increase the m RNA levels of Cacna2d1（P〈0.05）. Conclusion It is preliminarily verified that Cacna2d1 might be a target gene of mmu-mi R-107.