在富含CO2的厌氧环境下,产琥珀酸放线杆菌NJ113固定CO2合成丁二酸作为主要代谢终产物。在5L发酵罐探讨了培养条件对产琥珀酸放线杆菌NJ113固定CO2制备丁二酸的影响。考察了CO2供体形式、通气量、搅拌转速、培养温度和pH值对产琥珀酸放线杆菌NJ113厌氧发酵过程中CO2固定速率以及丁二酸产率的影响。结果表明,选择CO2气体作为C02供体,CO2通气量为0.75L/min,搅拌转速达到200r/min,培养温度为37℃,NaOH调节pH值为6.6,可增加细胞内可利用的CO2。在此优化条件下培养,CO2固定速率达到0.6g/(L·h),丁二酸产率达到1.61g/(L·h)。
Actinobacillus succinogenes NJ113 produces succinic acid as a major fermentation end product under CO2-rich anaerobic conditions. The culture conditions for succinic acid production by fixation CO2 with Actinobacillus succinogenes NJ113 were studied in a 5L fermentor. The effects of CO2 donor, CO2 flow rate, agitation rate, culture temperature and pH value on CO2 fixation rate and succinic acid productivity were investigated. The results show that when CO2 gas is as CO2 donor, CO2 flow rate is 0.75 L/min, agitation rate is 200 r/min, culture temperature is 37℃, pH value is 6.6, intracellular CO2 availability is improved. Under the optimized conditions, CO2 fixation rate can reach 0.6 g/(L· h) with succinic acid productivity of 1.61 g/(L·h).