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中文摘要：
对于以纤维素类原料（玉米皮）水解糖液作碳源厌氧发酵制备丁二酸的发酵液，利用Aminex HPX-87H（300mm×7．8mm i．d．，5μm）离子排斥色谱柱及折光示差检测器检测，以0．005mol／LH2SO4溶液（pH2．0）作流动相，在流速为0．6mL／min，柱温为55℃时对发酵液进行检测，16min内能将发酵液中甲酸、乙酸、丁二酸、葡萄糖、木糖、阿拉伯糖完全分离定量。6种物质线性相关系数均大于0．999，方法的回收率为99．3％-102．1％：RSD为0．6％～1．6％。本方法能够简便、快速测定丁二酸发酵体系中有机酸及单糖的含量。
英文摘要：
In order to determine the organic acids and monomeric sugars in fermentation broth with the cornhusk hydrolysate as carbon source, aminex HPX-87H ion exclusion column was used with refractive index detector. The mobile phase was 0. 005 mol/L of sulfuric acid （pH 2.0） with flow rate of 0. 6 mL/min at 55 ℃. Organic acids and monomeric sugars in samples were completely separated within 16 min. All the correlation coefficients（r） of regression equations were above 0. 999, and the recovery ranged from 99.3% to 102.1%. The relative standard deviations were in the range of 0. 6% -1.6%. By this method, organic acids and monomeric sugars can be easily, rapidly and effectively determined.
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