中文摘要：

采用Plackett—Burman设计法（Plackett—Burman，PB），对影响ActinobacillussuccinogenesNJ113厌氧发酵生产丁二酸的11个因子进行了筛选。结果表明，影响该菌厌氧发酵产丁二酸的主要因子为葡萄糖、酵母膏、玉米浆。在此基础上，采用响应曲面法（ResponseSurfaceMethodology，RSM）对这3个因子的影响进行了研究，得出丁二酸产量的数学模型，通过对二次多项回归方程求解，得到3因子的最优用量：葡萄糖107g／L，酵母膏16g／L，玉米浆12g／L，在优化条件下培养48h，丁二酸的产量由原始培养条件下的62g／L增到84g／L，收率从62％提高到78．5％，生产强度达1．75g／（L·h）。

英文摘要：

Plackett-Burman design was used to evaluate the effects of the eleven factors which influence the production of succinic acid by anaerobic fermentation of Actinobacillus succinogenes NJ113. The significant ones among the eleven factors were determined as follows： glucose, yeast extract and corn steep liquid （CSL）. The response surface methodology （RSM） was used to optimize the three factors. By solving the quadratic regression model equation, the optimal conditions of these variables were determined as： glucose 107g/L, yeast extract 16g/L, CSL 12g/L. After culturing under the optimized condition for 48 hours, the concentration of succinic acid increased from original 62g/L to 84g/L, the yield increased from original 62% to 78.5% with the productivity of 1.75 g/（L · h）.