中文摘要：

目的：探讨桃红四物汤对大鼠心梗后左室非梗死区心肌间质胶原重构的影响。方法：雄性Wistar大鼠,结扎左冠状动脉主干造成心肌梗死模型,将造模成功的大鼠随机分成3组：模型对照组、桃红四物汤组、卡托普利组;加上假手术组,共4组,每组12只。桃红四物汤组4.86 g·kg-1ig;卡托普利组0.2 g·kg-1自由饮水给药。假手术组和模型组给予同等剂量生理盐水,连续给药12周,12周后处死大鼠。采用Masson染色法检测心肌间质胶原纤维的表达情况,采用免疫组化法检测左室非梗死区心肌间质中Ⅰ型、Ⅲ型胶原蛋白的表达及Ⅰ/Ⅲ型胶原蛋白的比值。结果：桃红四物汤组大鼠心肌间质胶原纤维分布比较均匀、纤细,心肌间质纤维化程度较模型对照组明显减轻。模型组大鼠心肌间质胶原蛋白含量及Ⅰ/Ⅲ型胶原蛋白比值较假手术组明显升高,桃红四物汤组及卡托普利组的心肌间质胶原蛋白含量及Ⅰ/Ⅲ型胶原蛋白比值较模型组明显降低。结论：桃红四物汤可抑制大鼠心梗后心肌间质胶原纤维增生、胶原蛋白表达,降低非梗死区心肌Ⅰ/Ⅲ胶原蛋白的比值,减轻心肌间质胶原重构。

英文摘要：

Objective：To investigate the effects of Taohong Siwu Decoction on myocardium interstitial collagen reconstitution in the non-infarction regions of left ventricle in rats after acute myocardial infarction.Method： Male Wistar SPF rats were used.The ventricle remodeling was induced by ligating the left main coronary artery.Ventricle remodeling rats were divided into 4 groups：Model group,Taohong Siwu Decoction group,captopril group,sham group（n=12,each）.Taohong Siwu Decoction group was orally given the decoction of 4.86 g·kg-1.Captopril group was given captopril suspension at 0.2 g·kg-1 with free drinking of water.Sham group and model control groups were given the same volume of drinking water.All the rats were continuously treated for 12 weeks.Afterwards,the rats were killed,the myocardial tissues were sampled and then pathologically observed through Masson trichrome staining.The proteins of myocardial tissue collagen Ⅰand collagen Ⅲ were determined through immunohistochemical methods.Result： Myocardial myofibril and myofilament in Taohong Siwu Decoction group were in good order,normal morphology,evenly and thinly distributed collagenous fibers,myocardial mesenchyme fibrosis obviously decreased compared with those in the model comparison group.The content of myocardium collagen and the ratio of myocardial collagen type Ⅰto Ⅲ collagen protein in model control group were obviously higher than that in the sham operation group.The content of myocardium collagen and the ratio of myocardial collagen type Ⅰto Ⅲ collagen protein in Taohong Siwu group and captopril group were lower than those in model control group.Conclusion： Taohong Siwu Decoction can inhibit proliferation of myocardium interstitial collagenous fibers and expression of collagen protein after acute myocardial infarction in rats,cut down the ratio of myocardial collagen type Ⅰto Ⅲ collagen protein in the non-infarction regions of left ventricle,relief collagen reconstitution.