中文摘要：

目的：观察小儿推拿在小儿哮喘慢性持续期临床疗效和对肥大细胞TLR1、TLR2、TLR4表达变化影响。方法：将慢性持续期哮喘患儿160例,随机分为治疗组80例和对照组80例。对照组为常规雾化治疗,治疗组在对照组治疗的基础上,应用小儿推拿,各观察3个月,在治疗前后观察哮喘发作次数、呼吸道感染次数、C-ACT评分和PEF%并取外周血,检测肥大细胞TLR1、TLR2、TLR4。结果：治疗后治疗组哮喘发作次数、呼吸道感染次数、C-ACT评分、PEF%和肥大细胞TLR1、TLR2、TLR4表达荧光强度与对照组比较,有统计学差异（P〈0.05）,治疗组优于对照组。结论：小儿推拿手法可能通过上调肥大细胞TLR1、TLR2、TLR4表达,来改善小儿哮喘的临床症状。

英文摘要：

Objective： To investigate the effects of massage on pediatric asthma in chronic persistant stage and to observe the effect of the treatment on the TLR1, TLR2, TLR4 expression in the mast cells. Methods： One hundred and sixty patients in chronic persistant stage were enrolled and divided into two groups randomly, with 80 cases in each group. The control group received conventional atomization treatment, while the treatment group received with traditional techniques of pediatric massage based on conventional prevention treatment. All patients were assessed with the frequency of asthma attack and respiratory infection, C-ACT, and PEF% at the beginning of the training and 3 months after treatment. TLR1, TLR2, TLR4 expression in mast cells was also tested. Results： The content of TLR1, TLR2 and TLR4 in mast cells, the frequency of asthma attact and respiratory infection, C-ACT improved in after treatment. Compared with control group, there was significant difference in the treatment group （P〈0.05）. Conclusion： Massage might improve the clinical symptoms of pediatric asthma by ascending the content of TLR1, TLR2, TLR4 in mast cells.