中文摘要：

目的：建立制何首乌饮片HPLC特征图谱,为制何首乌饮片鉴别提供可靠方法。方法：采用HPLC色谱法,AQ-C（18）色谱柱（4.6 mm×250mm,5μm）,甲醇-0.05%磷酸水为流动相梯度洗脱,流速0.8 mL·min^-1,检测波长260 nm,柱温30℃。运用中药色谱指纹图谱相似度评价系统进行分析。结果：以2,3,5,4’-四羟基二苯乙烯-2-O-β-D葡萄糖苷为参照峰,初步构建了由4个特征峰组成的制何首乌饮片HPLC特征图谱。结论：该法建立的特征图谱可为制何首乌饮片的鉴别提供参考。

英文摘要：

Objective Establishing the HPLC specific chromatograms of the processed Polygonum multiflorum was a reliable method to identify the processed Polygonum multiflorum pieces. Methods the HPLC specific chromatography was adopted, with AQ-C（18）（4.6 mm × 250 mm, 5 μm） as the chromatographic column, with a gradient elution compose of methanol and 0.05% phosphoric acid at flow rate of 0.8 mL·min^-1, the detection wave length was 260 nm, the temperature of chromatographic column was 30 ℃. Using the similarity evaluation system of chromatographic fingerprint of traditional Chinese Medicine was to analysis data. Results Taking trans-2,3,5,4＇-tetrahydroxylstilbene-2-O-β-D-glucoside as the reference peak and establishing HPLC fingerprint of the processed Polygonum multiflorum pieces decoction which including 4 characteristic peaks. Conclusion this way of established HPLC specific chromatograms could provide the reference to identify the processed Polygonum multiflorum pieces.