中文摘要：

目的：优选槟榔标准饮片的均匀化方法，并建立一种同时测定槟榔饮片中4种生物碱含量的HPLC分析方法。方法：采用HPLC方法同时测定槟榔标准饮片中4种生物碱的含量，采用正交设计综合评分法，以出粉率、水溶性浸出物、粉末径距以及4种生物碱含量为参考指标，优化槟榔标准饮片的单次粉碎时间、粉碎次数及投料量，从而确定均匀化方法。结果：优化所得均匀化方法为每次投料150g，粉碎2次，单次粉碎时间50s。4种生物碱成分含量呈良好的线性关系，回收率分别为：去甲槟榔碱98.69%、槟榔次碱105.74%、去甲槟榔次碱99.48%、槟榔碱100.00%。结论：优化所得槟榔标准饮片均匀化方法合理稳定，建立的同时测定槟榔饮片中4种生物碱的含量的方法稳定可行。

英文摘要：

Objective ： To optimize the homogenization method of a traditional Chinese medicine Percarpium Arecae standard pieces , and establish an HPI.C method to simultaneously determine four alkaloids in it. Methods： Using HPLC technology determine the content of arecoline,arecaidine,guvcaoidine and guvacoline,then make the net content of 4 alkoids reference index, combined it with the content of flour yield,water extract,span,and apply the orthogonal design to optimize the homogenization of Percaroium Arecae standard pieces. Results： The homogenization method was A2B1C2 with crushing 150g materrials 2 times, and smashing 50s each time . The HPLC method showed good linearity for the exmined concentration ranges for the four alkoids,and the average recoveries of arecaidine.guvcaoidine and guvacoline.arecoline were 98.69%.105. 74%.99.48%. 100.00%. Conclusion： The homogenization method is stable and feasible for the Percarpium Arecae standard pieces,and the method has been successfully applied to determine four alkoids in it.