中文摘要：

适体核酶型核糖开关是近年出现的一种人工基因调控开关.最常见的适体核酶由锤头状核酶和适体组成,结构清晰,易于设计.作为一种顺式作用元件,适体核酶型核糖开关在特异性配体的作用下,无需蛋白质辅助,即可通过调节自身裂解反应,调控mRNA的翻译,可应用于多种细胞的基因调控.目前适体核酶型核糖开关的设计,主要是通过合理组装核酶与适体元件,整合到mRNA后再进行功能筛选.该基因调控开关调节幅度大、响应迅速、调控方式简洁,有可能应用于体内传感器、基因治疗、生物处理器等多个领域.

英文摘要：

The artificial aptazyme based riboswitch has emerged as a powerful tool for regulating gene expression in recent years. The most common aptazyme consists of a hammerhead ribozyme and an aptamer, it is simply a piece of structured RNA and easy to design. As a cis-acting element, aptazyme riboswitch can recognize specific targets and exert effective influence on translation without the help of protein co-factor through irreversible self-cleavage in vivo, which makes it a versatile platform functions in various cells. The most popular approach to generate an aptazme based riboswitch is to rationally cojoin a ribozyme to an aptamer and screen with a high throughout method after integrated into mRNA. With considerable magnitude of on-off control and rapid induction as well as concise regulation, this riboswitch will have important application in in vivo sensing, gene therapy and biological processor.