中文摘要：

综述实时荧光定量PCR（qPCR）技术在重组腺相关病毒（rAAV）的基因组滴度测定中的应用成果，以及qPCR技术在rAAV质量控制过程中的应用，如感染滴度及rcAAV污染率的测定等．分析qPCR技术存在的适用范围窄、易产生系统性误差和易受rAAV杂质干扰等问题的原因，并探讨相关的解决策略．

英文摘要：

This paper reviews the application achievement of real-time quantitative PCR （qPCR） technology in the re- combinant adeno-associated virus genome titer determination and its application in rAAVs＇ quality control, including in- fectious titer and contamination rate of reAAV. The current several problems of qPCR technique, such as relatively nar- row application range, easy to cause systemic errors and be interfered by rAAV impurity, are analyzed and discussed on better countermeasures.