中文摘要：

应用免疫组织化学技术和图象分析方法对乏情期、怀孕期牦牛卵巢的促卵泡素受体（FSHR）、促黄体素受体（LHR）的表达特点进行了研究。结果表明,牦牛卵巢的皮质、髓质、颗粒层和膜内层、黄体中都分布有FSHR和LHR。且牦牛卵巢皮质、髓质中的FSHR光密度值在乏情期显著大于怀孕期（P〈0.05）;怀孕期无黄体侧卵巢颗粒层和膜内层中FSHR光密度值均大于乏情期和怀孕期有黄体侧（P〈0.05）。怀孕期有黄体侧卵巢皮质处LHR光密度值最大,乏情期次之,怀孕期无黄体侧最小,各组间差异显著（P〈0.05）;在怀孕期无黄体侧卵巢髓质处LHR光密度值显著小于怀孕期有黄体侧和乏情期（P〈0.05）;在乏情期、怀孕期无黄体侧和怀孕期有黄体卵巢颗粒层和膜内层处LHR光密度值之间差异均不显著（P〉0.05）。表明牦牛卵巢中FSHR和LHR随着生殖阶段的不同而变化。

英文摘要：

The expression characteristics of follicle stimulating hormone receptor and luteinizing hormone receptor in yak ovaries during the anestrus and pregnancy were investigated by immunohistochemical technique and the section image software analysis techniques. The results showed that FSHR and LHR are mainly distributed in cortex, medulla, stratum granulosum and theca interna, corpus luteum of ovaries. In cortex and medulla, the FSHR mean optical densities of yak ovaries during anestrus were significantly greater than those during pregnancy（P〈0.05）, respectively;in stratum granulosum and theca interna,FSHR mean optical densities of yak ovaries without corpus luteum during pregnancy were significantly greater than those during anestrus between the 3 groups（P〈0.05） ; In medulla,LHR mean optical density of yak ovary without corpus luteum during pregnancy were significantly smaller than those with corpus luteum during pregnancy （P〈0.05） ,respectively. In cortex,the LHR mean optical density of yak ovaries with corpus luteum during pregnancy was the largest and that of yak ovaries without corpus luteum during pregnancy was the smallest among 3 groups,there were significant luteum during pregnancy and anestrus（P〈 0.05） ;in stratum granulosum and theca interna,the LHR mean optical densities of yak ovaries were no significant difference between the 3 groups（P〉0.05）. The results indicated that FSHR and LHR in yak ovaries varied with the reproductive phases.