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中文摘要：
建立了一个集成DNA聚合和RNA转录过程,能够重复产生RNA适体片段,并结合孔雀石绿产生荧光信号的分子机器,并探索了此分子机器在检测DNA方面的应用.转录产生的大量孔雀石绿适体序列被释放到溶液中,并可以与孔雀石绿结合产生荧光,实现信号的放大.85μL体系中的聚合转录的最适条件为：DNA聚合酶10 IU（0.118 IU·μL-1）,RNA聚合酶60 IU（0.706 IU·μL-1）,反应时间为3h.在上述条件下,荧光信号随着引发DNA用量的增加而增大.
英文摘要：
DNA polymerization and RNA transcription were combined to repetitively generate the fragments of the RNA aptamer,which in turn bind with malachite green to produce fluorescence signal,and the application of such a mechanism in the detection of DNA is explored.The aptamer sequence binds with malachite green to produce fluorescence,which can be employed to quantify the initially inputted triggering DNA.The optimum conditions for DNA polymerization and RNA transcription in 85 μL：DNA polymerase 1 μL,RNA polymerase 1 μL,the reaction time of 3 h.Under these conditions,the fluorescence signal increases with the increase of the amount of the trigger DNA.
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