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中文摘要：
目的：研究磁性纳米Fe304颗粒（MNP-Fe304）对藤黄酸（GA）诱导肝癌HepG2细胞凋亡的作用。方法将HepG2细胞分为GA0．5μmol／L单药组（A组）、GA0．5μmol／L和MNP-Fe3O4 20μg／ml两药联合组（B组）及空白对照组（C组）。采用MTT法检测HepG2细胞增殖的抑制率，流式细胞术检测细胞的凋亡率，Western blot法检测B细胞淋巴瘤／白血病2（Beb2）及半胱天冬氨酸蛋白酶3（Caspase-3）蛋白的表达。结果GA对HepG2细胞生长的抑制作用呈剂量依赖性。与C组相比，A、B组HepG2细胞凋亡率、Caspase-3蛋白表达增加，Bcl-2蛋白表达减少（P〈0．05），且B组各指标变化更为显著（Pd0．05）。结论MNP-Fe。04能增强GA对肝癌HepG2细胞的凋亡诱导作用；其机制可能与Bc1-2表达下调及Caspase-3表达上调有关。
英文摘要：
Objective To investigate the effects of magnetic nanoparticle Fe3O4 （MNP-Fe3O4） on apoptosis of hepatocellular carcinoma HepG2 cells induced by gambogic acid （GA）. Methods HepG2 cells were divided into three groups of A （treated with GA 0. 5 μmol/L）, B （treated with GA 0. 5 μmol/L plus MNP-Fe3O4 20μg/rnl） and C（blank controls）. The inhibitory rate of cell proliferation and the cell apoptosis were analyzed by MTT assay and flow cytometry, respectively. The protein expressions of B cell lymphoma/leukemia-2（Bcl-2） and cystein asparate proteinase-3 （Caspase-3） were detected by Western blot. Results GA had an inhibitory effect on proliferation of HepG2 cells in a dose-dependent manner. Compared with group C, the apoptosis of HepG2 cells and the protein expression of Caspase-3 were increased, while the protein expression of Bcl-2 was decreased in groups of A and B（P〈0. 05）, the changes of which were more significant in group B（P〉0. 05）. Conclusion MNP-Fe304 can promote GA-induced apoptosis of HepG2 cells, which may be related to down- regulating the expression of Bcl-2 and up-regulating the expression of Caspase-3.
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