中文摘要：

用纤维素DE-52柱层析和硫酸铵分级分离法快速分离，纯化获得CF1-ATP合酶，聚丙烯酰胺凝胶电泳技术对其纯度和凝胶内酶活性进行检测，研究了温度，pH值、盐浓度对CF1水解酶活性的影响，用原子力显微镜对CF1的形态结构进行了观察。结果发现，当pH值为8．0时，CF1可在0℃存放数天并保持其50％的活性，20％的甲醇溶液能够有效地保护酶分子的结构；实验还通过低温盐解作用获得了具有活性的亚基；用原子力显微镜观测显示CF1-ATP合酶分子平均大小约为18nm，按其直径大小可以分为三类，推测其分别对应着ATP合酶的三种状态，即空置态、疏松结合态和紧密结合态。

英文摘要：

CF1-ATPase was isolated and purified from spinach chloroplast by cellulose DE-52 chromatography and ammonium sulfate fractionation. Then its purity and in-gel activity were detected through PAGE. The effects of temperature, pH value and salt concentration on the activity of CF1-ATPase were also studied, and the topography of CF1-ATPase was observed by AFM. It was found that the activity kept 50% at 0℃ several days while pH value was 8.0, and that 20% methanol protected its molecule structure effectively. Besides, the authors obtained active subunits through incubating in 1 mol/L KC1 at 0℃. By AFM observation, the enzyme molecules were average 18 nm in diameter, and were sorted into 3 groups corresponding to three states （Omit state, Loose state and Tight state） of the enzyme respectively.