中文摘要：

目的探讨不同化疗栓塞方式对肝细胞癌（HCC）血管生成的影响。方法搜集手术切除后病理证实的HCC标本136例，单纯手术57例（A组），灌注化疗＋手术11例（B组），经导管动脉化疗栓塞（TACE）＋手术68例（TACE组）。TACE组按栓塞方式又分为：化疗药＋碘油33例（C组），化疗药＋碘油＋明胶海绵23例（D组），化疗药＋碘油＋无水乙醇＋明胶海绵12例（E组）。按手术切除距末次栓塞时间分为≤30d组（24例）、31—60d组（25例）、61—90d组（13例）、〉90d组（6例）。标本采用免疫组织化学法染色，并检测各组CD31和血管内皮生长因子（VEGF）的表达情况，对CD31阳性血管内皮进行微血管密度（MVD）计数。结果MVD：A、B、TACE组残余肿瘤组织平均MVD分别为（44±18）、（48±23）、（55±22）个／高倍视野，A组与B组比较，差异无统计学意义（t=-0．057，P〉0．05）；TACE组高于A组（t=-2．897，P〈0．05）；TACE组（C组、D组、E组）残余肿瘤组织平均MVD分别为（56±22）、（54±24）、（52±19）个／高倍视野，各TACE组之间（C组、D组、E组）比较差异无统计学意义（F=0．152，P〉0．05）。≤30d组、31—60d组、61—90d组、〉90d组平均MVD分别为（50±19）、（554-24）、（624-29）、（534-10）个／高倍视野，均比A组高（假设A组为TACE术后第0天所采集的标本，以此作为对照组），但≤30d组（t=一1．39，P〉0．05）和〉90d组（t=-1．11，P〉0．05）差异无统计学意义；31—60d组（t=-2．06，P〈0．05）和61—90d组（t=-2．19，P〈0．05）差异有统计学意义。VEGF：A组、B组、TACE组比较，残癌组织中VEGF表达强度依次增加，差异有统计学意义（X2=12．629，P〈0．05），TACE各亚组（C组、D组、E组）之间比较，差异无统计学意义（X2=2．310，P〉0．05）。对所有标本VEGF表达强度与MVD值进行Pearson相关分析，随着VEGF

英文摘要：

[Abstract ] Objective To investigate the effect of different methods of transarterial chemoembolisation （TACE） on the angiogenesis of hepatocellular carcinoma （HCC）. Methods One hundred and thirty-six HCC specimens from 136 patients were collected. Fifty-seven tumors came from patients who were treated only by surgery （group A ）, 11 tumors from patients who were treated by transarterial chemoperfusion before surgery （group B）, 68 tumors from patients who were treated by TACE before surgery （group TACE ）, respectively. And the TACE group was classified into 3 sub-groups： iodinated oil ＋ chemotherapeutics for 33 cases （group C）, iodinated oil ＋ chemotherapeutics ＋gelatin sponge for 23 cases （group D） ,iodinated oil ＋ chemotherapeutics ＋gelatin sponge ＋ absolute alcohol for 12 cases （group E）, respectively. Also, the tumors of TACE were divided into 4 groups according to an elapse between the last TACE therapy and the surgery. They were group ≤30 days （n = 24）, group 31-- 60 days （ n = 25）, group 61---90 days （ n = 13 ）, group 〉 90 days （ n = 6）, respectively. The pathological sections were stained by immunohistochemical method. The microvessels were marked by CD31. And the stained cells were counted under the light microscope. Results The mean of microvessels density（MVD） were（44 ± 18 ）, （48 ± 23 ） , （55± 22 ） , respectively. The mean of MVD in group TACE was significantly higher than that in group A （ t = - 2. 897, P 〈 0. 05 ）. There was no significant difference for the mean of MVD between group A and group B （t = - 0. 057, P 〉 0. 05 ）. The mean of MVD in group C, group D and group E were （56 ± 22）, （ 54 ± 24 ）, （ 52 ± 19） , respictively. The differences of the mean of MVD were not statistically significant among the three groups. （ F = 0. 152, P 〉 0. 05 ） . The mean of MVD in group ≤30 days,group 31---60 days,group 61----90 days,group 〉90 days were （50 ± 19）, （55 ±24） , （62 ±29?