中文摘要：

目的：筛选土家药延龄草中抗SH-SY5Y细胞谷氨酸（Glu）损伤的有效部位。方法：Bliss法测定延龄草不同部位提取物的1%致死量（LD1）和半数致死量（LD50）,建立Glu损伤SH-SY5Y细胞模型,筛选延龄草中抗SH-SY5Y细胞Glu损伤的有效部位,以形态学、细胞存活率和LDH漏出率为指标评价延龄草有效部位对Glu损伤细胞的保护作用。结果：TTM1为抗SH-SY5Y细胞Glu损伤的有效部位,能显著改善Glu损伤细胞的形态,5.0、10.0、20.0μg/mL的TTM1提高损伤细胞的存活率（P〈0.05,P〈0.001）,2.5、5.0、10.0、20.0μg/mL的TTM1可显著降低损伤细胞的LDH漏出率（P〈0.05,P〈0.001）。结论：延龄草TTM1对Glu损伤的SH-SY5Y细胞具有保护作用。

英文摘要：

Objective： To screen the effective parts of Tujia medicine birthroot against the damage of glusate（Glu） in SH-SY5Y cells. Methods： Bliss method was used to test the LD1 and LD50 of birthroot＇s dif erent extractives. The SH-SY5Y cells model induced by Glu was established, and effective parts against the Glu damage in SH-SY5Y cells of birthroot were screened. The protective effect of the effective part of birthroot was evaluated by the survival rate, LDH leakage rate and morphology changes. Results： TTM1 was the effective part of birthroot, which could protect the SH-SY5Y cells against Glu injury. The survival rate of damaged cells could be improved by 5.0, 10.0 and 20.0μg/mL TTM1（P〈0.05, P〈0.001）. The LDH leakage rate of damaged cells could be reduced signii cantly by 2.5, 5.0, 10.0 and 20.0μg/mL TTM1（P〈0.05, P〈0.001）. Conclusion： TTM1 of birthroot could protect the SH-SY5Y cells damaged by Glu.