本试验旨在研究黄褐毛忍冬提取物对小鼠生长性能、腹泻率和抗氧化性能的影响.将90只小鼠随机分为空白组,试验Ⅰ、Ⅰ、Ⅲ组及对照组,空白组饲喂基础日粮,试验组在基础日粮中分别添加0.02、0.04及0.06 mg/g体重的黄褐毛忍冬提取物,对照组则在基础日粮中添加硫酸抗敌素,并通过小鼠番泻叶灌服法,观察其腹泻情况.结果显示,试验Ⅱ、Ⅲ组及对照组小鼠末重均显著高于空白组(P<0.05);黄褐毛忍冬提取物对试验组小鼠的生长性能均有所影响,但对小鼠肝脏增重无显著影响(P>0.05);试验组小鼠腹泻率随提取物浓度的增加而降低,对照组小鼠腹泻率显著低于空白组(P<0.05).与空白组相比,各试验组(除Ⅰ组外)小鼠血清谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性均有所提高,且试验Ⅲ组小鼠血清超氧化物歧化酶(SOD)活性较空白组显著提高(P<0.05).结果表明,日粮中添加黄褐毛忍冬提取物能够提高小鼠的生长性能与抗氧化性能,降低小鼠的腹泻率,不同浓度的提取物产生的效果有差异,其中以添加0.06 mg/g提取物效果最佳.
The study was carried out to study the effects of Lonicera fulvotomentosa extract on growth performance,diarrhea rate and anti-oxidation performance in mice.90 mice were randomly divided into five groups:Blank group,experimental groups Ⅰ,Ⅱ,Ⅲ and control group.The blank group was fed with basal diet,the experimental groups were added the 0.02,0.04 and 0.06 mg/g BW of Lonicera fulvotomentosa extract to basal diet,and the diet of control group was added the colistin sulfate observing the diarrhea rate in mice with folium sennae perfusion.The results showed that comparing with blank group,the finial weight of mice in experimental groups Ⅱ,Ⅲ and control group were significantly improved (P<0.05);The Lonicera fulvotomentosa extract had effects on growth performance in the experimental groups,there was no significant effects on the liver weight (P<0.05);The diarrhea rate in the experimental groups were decreased with the increase of the concentration of Lonicera fulvotomentosa extract,and the diarrhea rate in the control group was significantly lower than blank group (P<0.05).Compared with the blank group,the activity of GSH-Px and CAT in experimental groups (except group Ⅰ) were increased,and the serum SOD activity in experimental group Ⅲ was significantly increased (P<0.05).In conclusion,the growth performance and anti-oxidation performance of mice were increased while the diarrhea rate was decreased by adding the Lonicera fulvotomentosa extract,and adding 0.06 mg/g BW concentration had the best effects.