中文摘要：

用紫外-可见光吸收光谱、荧光发射光谱法研究牛血清白蛋白与柠檬黄和日落黄之间的相互作用;用SternVolmer方程处理实验数据,得到310 K时的动态猝灭速率常数（KQ）分别为2.51×105 L/mol和1.42×105 L/mol;用Lineweaver-Burk双倒数函数方程处理实验数据,得到310 K时静态猝灭常数（KLB）依次为5.79×105 L/mol和5.56×105 L/mol;用lg（（F0-F）/F）=lgK0＋nlgρ处理实验数据,得到结合位点数（n）分别为0.556 95、0.640 56。并且两种色素在质量浓度为0~3μg/mL范围内其含量与荧光猝灭强度具有良好的线性关系。

英文摘要：

Interactions between bovine serum albumin （BSA） and lemon yellow or sunset yellow were studied by UV-visible absorption spectroscopy and fluorescence emission spectroscopy. The dynamic quenching rate constants at 310 K were calculated according to Stem-Volmer equation to be 2.51 × 10^5 and 1.42 × 10^5 L/mol for lemon yellow and sunset yellow, respectively. The static quenching constants at 310 K were calculated according to Lineweaver-Burk double-reciprocal equation to be 5.79 × 10^5 and 5.56 × 10^5 L/mol, respectively. The number of binding sites at 310 K was calculated according to lg（（Fo--F）/F）=lgKo＋nlgp to be 0.556 95 and 0.640 56, respectively. A good linear relationship was observed between lemon yellow or sunset yellow concentration in the range of 0 - 3 μg/mL and fluorescence quenching intensity.