中文摘要：

以月桂酸三甘油酯和甘油为原料,脂肪酶催化甘油解反应合成月桂酸单甘油酯。在单因素试验的基础上,采用响应面分析法进行合成工艺优化。结果表明,含水量（相对于甘油的质量）对月桂酸单甘油酯产率的影响最为显著,且较优合成条件为：恒温振荡器转速100 r/min,酶添加质量分数（相对于底物）5%,n（甘油）∶n（月桂酸三甘油酯）=6∶1,底物质量分数（相对于整个反应体系）51.9%,温度65℃,含水量4.23%,反应时间5 h。在此条件下,月桂酸单甘油酯产率的预测值和实验值分别为81.68%和81.32%,说明二次多项回归模型具有良好的预测性。

英文摘要：

Monolaurin was prepared by glycerolysis of trilaurin with lipase as the catalyst and t- butanol as solvent. Optimization of the reaction process was conducted based upon single factor tests and adopting response surface methodology. The results showed that water content（ mass fraction referring to glycerol） is the most significant factor on the yield of monolaurin. The suitable conditions are as follows： rotation speed of the oscillator,100 r / min; enzyme dosage,5%（ based on the amount of total substrates,trilaurin and glycerol）;molar ratio（ glycerol to trilaurin）,6 ∶ 1; mass fraction of the total substrates,51. 9%（ referring to the total amount of all reactants and solvent）; reaction temperature,65 ℃; water content,4. 23%; and reaction time,5 h. The experimental yield of monolaurin achieves 81. 32% under the aforesaid conditions,which is well in conformity with the predicted value of 81. 68%. Therefore,the regression model shows good predictability.