中文摘要：

目的：观察Ca^2＋/PKC通路在大鼠心肌细胞缺氧复氧状态下参与Egr-1高表述。方法：取生长5～6d的大鼠心肌细胞分为对照（Control）组、缺氧复氧（H／R）组、溶剂（DMSo）组、EGTA组、维拉帕米（VER）组、H7组和BIM组。各组心肌细胞制作H/R模型。RT-PCR法检测培养心肌细胞中Egr-1mRNA的表达水平。Western-blot法检测培养心肌细胞中Egr-1蛋白的表达水平。结果：与Control组相比，H/R组及DMSO组心肌细胞Egr-1mRNA的表达水平明显增高（P〈0.05）；与H/R组相比，VER组、EGTA组、H7组、BIM组Egr-1mRNA的表达明显下调，差异具有统计学意义（P〈0.05）。与Contr01组相比，H/R组及DMSO组心肌细胞Egr-1蛋白表达水平明显增高（P〈0.05）；与H/R组相比，VER组、EGTA组、H7组、BIM组Egr-1蛋白的表达明显下调，差异具有统计学意义（P〈0.05）。结论：在大鼠心肌细胞缺氧复氧状态下，Ca”/PKC信号通路参与介导了Egr-1的高表达。

英文摘要：

AIM： To study effects of Ca^2＋/PKC pathway on Egr-1 overexpression in hypox-ia/reoxygenation cardiomyocytes of rats. METHODS： Cultured cardiomyocytes of 5-6 day old rats were divided into control group, hypoxi a/reoxygenation （H/R） group, DMSO group,EGTA group , Verapamil BIM group. All groups group, of cardi H7 group and omyocytes derwent the treatment of H/R, and then un RT PCR and Western blot were used to test expres- sion of Egr-1 mRNA and Egr-1 protein respec-tively. RESULTS-There were high expression of Egr-1 mRNA in H/R group and DMSO group compared with control group （P〈0.05）. Vera-pamil, EGTA, H7 and Bim reduced the expres- sion of Egr-1 mRNA significantly compared with H/R group （P〈0.05）. There were high ex- pression of Egr-1 protein in H/R group and DM- SO group compared with control group （P〈0.05）. Verapamil, EGTA, H7 and Bim reduced the expression of Egr-1 protein significantly compared with H/R group （P〈0.05）. CON-CLUSION： Ca2t/PKC pathway plays a role on Egr-1 overexpression in hypoxia/reoxygenation cardiomyocytes of rats.