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中文摘要：
建立了高效毛细管电泳-电喷雾飞行时间质谱联用（HPCE—ESI—TOF／MS）快速定性分析黄连中生物碱类化合物的分析方法．使用未涂层石英毛细管，以50mmol／L 乙酸铵-0．5％甲醇溶液（用氨水调至pH=7．2）作为运行缓冲液，分离电压为25kV；鞘液组成为50％甲醇-49．5％水-0．5％乙酸，鞘液流速为4μL/mn；质谱选用正离子模式，碰撞电压（Fragmentor）为100V．结果表明，通过各色谱峰紫外光谱和质谱测得精确分子量结果，结合文献，对黄连中7种生物碱进行了鉴定．表明本方法简便、快速，是黄连中生物碱类化合物快速分离、鉴别的有效方法．
英文摘要：
A new method for the analysis of alkaloids in Coptis chinensis Franch was established by high performance capillary electrophoresis-electrospray ionization time of flight mass spectrometry （HPCE-ESI-TOF/MS）. The real samples were separated by an uncoated capillary. 50 mmol/L ammonium acetate containing 0.5% methanol （pH=7.2） was used as the running buffer, and separation voltage was 25 kV. A coaxial sheath flow interface was used as the CE-MS interface, and a 50% methanol-49.5% water- 0.5% acetic acid mixture was used as the sheath liquid with a flow rate of 4 μL/min. The lens voltages in a positive ion mode with a collision induced dissociation （CID） voltage of 100 V were used for ESI-TOF/MS analysis. Seven alkaloids in Coptis chinensis Franch methanol extracts were separated and identified by CE-DAD and CE-ESI-TOF/MS. The coupling of HPCE separation with accurate mass measurement capability of ESI-TOF/MS provides an attractive tool for the identification of alkaloid compounds in Coptis chinensis Franch.
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