中文摘要：

目的：研究建立大黄不同来源药材中芦荟大黄素-8-O-β-D-葡萄糖苷和大黄酸-8-O-β-D-葡萄糖苷2个蒽醌苷类成分的含量测定方法。方法：采用HPLC法同时测定2个蒽醌苷类成分含量，使用Agilent TC-C18（2）柱（4.6 mm×250 mm，5 μm），流动相为乙腈-1%醋酸溶液（20∶80），流速1.0 mL·min-1，检测波长410 nm，柱温35 ℃。结果：芦荟大黄素-8-O-β-D-葡萄糖苷和大黄酸-8-O-β-D-葡萄糖苷进样量分别在0.02-0.35 μg（r=0.9998,n=5）,0.08-1.68 μg（r=0.9999,n=5）范围内线性关系较好，平均回收率（n=6）分别为97.2%和98.9%。结论：所建立的方法可用于同时测定大黄药材中2个蒽醌苷的含量，为大黄药材质量评价标准的制定提供了科学依据。

英文摘要：

Objective：To establish the quantitative method of two anthraquinone-O-glucopyranosides（aleo-emodin-8-O-β-D-glucopyranoside,rhein-8-O-β-D-glucopyranoside） in different botanical origins of rhubarb.Methods：The separation of two anthraquinone-O-glucosides were determined simultaneously by HPLC on an Agilent TC-C18（2）column（4.6 mm×250 mm,5 μm） at 35 ℃ with the acetonitrile-1% acetic acid（20∶80）.The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 410 nm.Results：The standard curves for aleo-emodin-8-O-β-D-glucopyranoside,rhein-8-O-β-D-glucopyranoside were linear in the ranges of 0.02-0.35 μg（r=0.9998,n=5） and 0.08-1.69 μg（r=0.9999,n=5）;The average recoveries（n=6） were 97.2% and 98.9%,respectively.Conclusion：The established method has provided a scientific basis for the quality evaluation,and can be used to the quality control of rhubarb.