中文摘要：

目的：探讨针灸对肾阳虚证骨关节炎患者免疫相关基因表达的影响.方法：用基因芯片技术检测肾阳虚证骨关节炎患者及正常人的基因表达谱,以及针灸治疗显效者的治疗前后基因表达谱的变化.结果：通过基因表达谱芯片筛选出与肾阳虚骨关节炎免疫相关的基因13条,肾阳虚骨关节炎患者与正常人相比表达下调4条,上调5条.经温针治疗显效的4例患者治疗前后的基因芯片比较结果显示：NM000265、NM001838基因普遍上调,NM_001784基因普遍下调,其他基因表达无规律性变化.结论：肾阳虚证骨关节炎的发生涉及免疫相关基因表达的异常,针灸可能通过诱导或阻遏这些基因的表达发挥效应.

英文摘要：

Objective ： To explore the effect of acupuncture on expression of immuno - related genes in patients of Kidney - Yang Deficiency Osteoarthritis. Methods：The gene expression profile in patients of Kidney - Yang Deficiency Osteoarthritis and normal subjects was assayed by gene microarray, and the changes of gene expression profile in the pro - treat and post - treat of the typical cases that they are very effective through acupuncture were also measured. Results ： 13 immuno - related genes in patients of Kidney - Yang Deficiency Osteoarthritis were screened with gene chip technique,including 5 genes up regulating and 4 genes down regulating in patients,as compared with normal subjects. The gene chip results of 4 typical cases that they are effective through acupuncture showed that NM000265, NM001838 were up - regulated and NM 001784 was down- regulated. Conclusion：The pathogenesis of Kid- ney -Yang Deficiency Osteoarthritis may be involved in the abnormal expression of immuno - related genes. It is suggested that acupuncture may exert its effect by way of inducing or repressing the expression of these genes.