目的比较甲醛熏蒸、三氧消毒机两种消毒方法对血库冰箱空气的消毒效果。方法甲醛熏蒸法是按40mL/m^3用量,紧闭冰箱门,熏蒸2h;三氧消毒机消毒法是紧闭冰箱门开机2h,停机40min后使用。消毒前、后进行2次空气采样,培养48h,进行细菌菌落计数。结果两种消毒方法消毒前、后普通细菌数量及真菌生长情况比较,差异有统计学意义(P〈0.01)。两种方法消毒后,细菌总数均在200cfu/m^3以下,但甲醛熏蒸存在毒性残留问题,而三氧消毒机消毒法对人体损害较小。结论三氧消毒机消毒法消毒效果较好,且安装方便,在消毒过程中可开冰箱取血、发血。
Objective To compare the efficiency of sterilization for blood bank refrigerator with the method of formaldehyde fumigating and tri-oxygen sterilizer. Methods The formaldehyde fumigating method was to fumigate the closed refrigerator with the 40 mL/m^3 flow for 2 h, The method of tri-oxygen sterilizer was to run for 2 h with door closed and to use it 40 min later since shutting off. And then air sample before and after sterilization was cultured for 48 h and the bacterial colony was counted. Results There were significant differences before and after sterilization in the bacterial number and the growing status of mycetes (P〈0.01). The total counts of bacteria were both below 200 cfu/m^3 with the two methods, but there was some residual of toxicity with the formaldehyde fumigating method and little with tri-oxygen sterilizer. Conclusion It is better to use tri-oxygen sterilizer method has better sterilization efficiency, which is convenient to install and take in or put out blood during sterilization.