中文摘要：

目的：观察赤雹根总皂苷对II型胶原性关节炎（CIA）模型大鼠的治疗作用及对CD4~＋、CD8~＋ T细胞表达的影响。方法：雄性Wistar大鼠80只,随机取10只作为正常对照组,其余大鼠尾根部多点皮内注射牛II型胶原和完全弗氏佐剂的乳化剂,7天后加强免疫一次,建立CIA大鼠模型,以关节炎指数（AI）≧6为建模成功。将模型大鼠按AI值水平随机分为赤雹根总皂苷80、40、20mg/kg组、雷公藤多苷12mg/kg组和模型对照组,每组11只。各给药组连续ig给药35天,模型对照组合正常对照组ig等体积的蒸馏水。分别于给药前及给药后7、14、21、28、35d测定大鼠四肢踝关节肿胀度和计算关节炎指数。末次给药后,取血清、脾脏,计算脾脏指数（SI）;ELISA及Western Blot法分别检测CIA大鼠血清及脾脏中T淋巴细胞亚群CD4~＋、CD8~＋ T细胞的蛋白表达水平。结果：给药第35d,赤雹根总皂苷80、40、20mg/kg组踝关节肿胀度、AI值、SI值均显著低于模型对照组;血清及脾脏中CD4~＋T细胞的蛋白表达水平、CD4~＋/CD8~＋的比值均显著低于模型对照组;CD8~＋ T细胞的蛋白表达水平均显著高于模型对照组。结论：赤雹根总皂苷对RA有较好的治疗作用,调节CD4~＋、CD8~＋ T淋巴细胞的水平是其治疗RA的主要机制之一。

英文摘要：

Objective： To observe the therapeutic effect of saponins from Thladiantha dubia Root（ TSTR） on arthritis（ CIA） rats induced by collagen and its effect on T lymphocyte subsets CD4~＋ and CD8~＋ T lymphocyte subset. Methods： 80 male Wistar rats were used,10 of them were randomly taken as the control group,and others were used to establish model rats. The emulsifier of mixture of bovine type II collagen and freund＇s complete adjuvant was intradermally injected into ends of rats＇ tails,then the immune function of model rats were strengthened 7 days after the injection. When the arthritis index（ AI） ≧ 6 in the rat,the rat was considered as the CIA rat. The model rats were divided according to AI into TSTR 80,40,20mg/kg groups,Tripterygium Glycosides 12mg/kg group and model group,with 11 rats in each group. Rats in groups were intragastriced（ ig） administered with corresponding drugs for 35 days,while rats in the model group and control group were ig given with distilled water. The swelling inhibition rate of ankles and the arthritis index of rats were calculated before administration and 7th,14 th,21st,28 th,35th day after ig administration. Serum and spleen were collected and the spleen index（ SI） was calculated after the last administration. The protein expressions of CD4~＋ ,CD8~＋ T cells of T lymphocyte subsets in serum and spleen of CIA rats were detected by ELISA and Western Blot. Results： The swelling inhibition rate of ankles,AI,SI in TSTR 80,40,20mg/kg were all significantly lower than those in model group（ P〈0. 01）; the protein expressions of CD4~＋ T cells in serum and spleen and CD4~＋ /CD8~＋were significantly lower than those in the model group; the protein expression of CD8~＋was significantly higher than that in model group（ P〈0. 01）. Conclusion： TSTR has the therapeutic effect on RA. Its regulation on T lymphocyte CD4~＋ and CD8~＋level is one of the main mechanisms.