中文摘要：

目的 探讨大熊猫肠道真菌菌群结构，为研究肠道真菌对纤维素的降解和控制肠道真菌病奠定基础。方法 采用PDA、SDA、高氏3种培养基对8只亚成体大熊猫新鲜粪便进行真菌分离、纯化，经形态学和分子生物学方法进行鉴定。结果 经培养获得的真菌主要由4种霉菌（Galactomyces geotrichum占22.74%、Galactomyces reessii占12.37%、Mucor ramosissimus占18.23%和Mucor circinelloides占6.39%）和2种酵母菌（Trichosporon sp. 占19.46%和Candida solani strain占20.81%）组成，各样品中真菌所占比例不等。且3种培养基的分离效果显示PDA〉SDA〉高氏培养基。结论 亚成体大熊猫肠道内存在一定比例的可培养真菌。

英文摘要：

Objective To investigate the fungal community structure in the guts of sub-adult giant pandas, and provide the basis for studying the intestinal fungi to digest cellulose and controlling the fungal disease. Methods In this study, three mediums such as PDA, SDA and Gao’s were chosen to isolate and purify the fungi in fresh feces of sub-adult giant pandas, followed by the combination of morphological and molecular methods for classification. Results The study showed that the fresh faces of eight sub-adult giant pandas were mainly composed of four kinds of mould （Galactomyces geotrichum, 22.74%; Galactomyces reessii, 12.37%; Mucor ramosissimus, 18.23%; Mucor circinelloides, 6.39%） and two kinds of yeast （Trichosporon sp., 19.46%; Candida solani, 20.81%）. The proportions of fungus were different among each sample. The isolation effect of three mediums was： PDA〉SDA〉Gao. Conclusions There are a proportion of culturable fungi in the guts of sub-adult giant pandas.