中文摘要：

为了解四川省成都市动物园野生动物贾第虫的流行及基因型，本研究采集了146份不同野生动物的新鲜粪便并提取基因组DNA．通过巢式PCR扩增β-giardin、tpi和gdh基因，扩增产物测序后进行种系发育分析．结果表明，CDZOO1粘鹿源和CDZOO3龟源贾第虫通过多位点基因分型（MLG）鉴定为AI-1亚型；CDZ002鹿源贾第虫为E型（β-giardin基因位点）；CDZOO4粘鹿源贾第虫为A型（β-giardin和tpi基因位点）；CDZOO5浣熊源和CDZOO6细尾艨源贾第虫在β-giardin位点为D型而在tpi位点为A型．

英文摘要：

In order to investigate the infection and genotypes of Giardia from different wildlife in Chengdu zoo, a total of 146 fresh fecal samples were collected and their genome DNA extracted. The β-giardin, tpi and gdh genes were amplified by nested-PCR and the product were sequenced followed by phylogenetic analysis. As a result, two persian fallows （CDZOO1 and CDZOO4）, a deer （CDZOO2）, a tortoise （CDZOO3）, a raccoon （CDZOO5）, a meerkat （CDZOO6） were infected with Giardia. Multilocus genotypes （MLGs） identified assemblages AI-1 in CDZOO1 and CDZOO3 ; CDZ002 was infected with as- semblage A in both β-giardin and tpi loci; CDZOO4 was confirmed as assemblage A at the tpi locus; CDZOO5 and CDZOO6 were identified as assemblage D at the β-giardin locus while assemblage A based on the tpi locus.