东篱科研大数据发现系统（DRDS）

位置：成果数据库 > 期刊 > 期刊详情页

^99mTc-Gd-DTPA-BMA的制备及其生物学分布研究

ISSN号：1002-266X
期刊名称：《山东医药》
时间：0
分类：R817[医药卫生—影像医学与核医学;医药卫生—放射医学;医药卫生—临床医学]
作者机构：[1]泸州医学院附属医院,四川泸州646000
相关基金：国家自然科学基金资助项目（30970858）; 四川高等学校科技创新重大培育项目（2009-07）; 四川省杰出青年学科带头人基金（2008-46-386）

关键词：钆双胺, 锝, 同位素标记, 生物学分布, gadodiamide, 99mTc, isotope labeling, bio-distribution

中文摘要：

目的以99m锝（99mTc）标记钆双胺（Gd-DTPA-BMA）制备99mTc-Gd-DTPA-BMA并对其基本生物学分布特征进行研究,旨在为SPECT-MRI双模式显像剂的应用提供依据。方法以氯化亚锡为还原剂,采用一步法以99mTc分别标记Gd-DTPA-BMA、二乙三胺五乙酸（DTPA）,纸层析法测定两标记物标记率、不同pH值及室温下观察稳定性;将99mTc-Gd-DTPA-BMA用生理盐水稀释后注射至小鼠尾静脉,记录注药后1~240 min每克组织百分注射剂量率（%ID/g）;取家兔3只耳缘静脉分别弹丸注射99mTc-Gd-DTPA-BMA和99mTc-DTPA,采用SPECT仪以肾动态显像模式行双时相采集,记录分肾摄取百分比（Uptake%）、达高峰时间（Tmax）、半排时间（T1/2）。结果 99mTc-Gd-DTPA-BMA标记率和稳定性均较高,通过静脉注入小鼠体内后主要分布于肾脏;99mTc-Gd-DTPA-BMA和99mTc-DTPA均经家兔肾脏排泄,其他脏器摄取率较低,但前者Tmax及T1/2显著大于后者（P〈0.05）。结论 99mTc标记Gd-DTPA-BMA具有标记率和稳定性较高等优点,所得99mTc-Gd-DTPA-BMA静脉注入体内后主要分布于肾脏并经肾脏排泄;此为SPECT-MRI双模式显像剂的制备及应用提供了依据。

英文摘要：

Objective To prepare 99mTc-Gd-DTPA-BMA and investigate its biologic characteristics,and to provide the basis of 99mTc-Gd-DTPA-BMA using as a SPECT-MRI dual-modality agent.Methods Both Gd-DTPA-BMA and DTPA were synthesized,labeled with 99mTc by a stannous chloride reduction method.The radiochemical purity was determined by paper chromatography.Its stability was observed at room temperature and different values of pH.The 99mTc-Gd-DTPA-BMA diluted with normal saline,was injected into mice intravenous.The percentage of the injected dose per gram of tissues mass（% ID/g） was measured at 1to 240 mins after injection;Three rabbits were injected 99mTc-Gd-DTPA-BMA and 99mTc-DTPA through auricular vein,and the dynamic scintigraphy in rabbits were performed by SPECT after injection,the uptake percentage（Uptake%）,time to peak（Tmax） and time from peak to 1/2 peak（T1/2） were observed respectively.Results Both labeling efficiency and stability of 99mTc-Gd-DTPA-BMA were high.The highest uptake after injection is kidney.Two labeled compounds were excreted by the kidney,but Tmax and T1/2 of 99mTc-Gd-DTPA-BMA were longer than 99mTc-DTPA（P0.05）.Other organs have a little distribution of radiopharmaceuticals.Conclusions The methods of 99mTc labeled Gd-DTPA-BMA have high labeling efficiency and stability.99mTc-Gd-DTPA-BMA was major excreted by the kidney.It is expected to provide the basic information for the further study of SPECT-MRI dual-modality imaging agent.

同期刊论文项目