目的:对人未成熟卵母细胞玻璃化冷存保存技术进行初步实验探讨。方法:采用OPS玻璃化冷冻方法,对未成熟的卵子进行冷冻,解冻后体外成熟培养,ICSI受精,观察胚胎培养情况,并对某些影响因素进行探讨。结果:冷冻了GV期卵62个,M I期卵40个。两期卵细胞复苏后存活率、成熟率、受精率和卵裂率分别为:80.65%、77.50%,52.00%、41.94%,57.69%、61.54%,60.00%、62.50%,两组间比较无统计学差异(P〉0.05);采用HTF或G-Mops两种缓冲液配制玻璃化冷冻液冷冻解冻未成熟卵效果无统计学差异(P〉0.05);未成熟卵解冻后置于含性激素的TCM199与单纯IVF30中成熟培养效果无统计学差异(P〉0.05)。结论:应用OPS玻璃化冷冻方法能够有效冻存未成熟卵母细胞。
Objective: To explore the efficiency of vitrification of hunan immature oocytes. Methods: The human immature oocytes were freezed by OPS vitrification method, then cultured in vitro after thawing, ICSI fecundation, culture conditions were observed and related factors were explored. Results: 62 GV and 40 MI oocytes were freezed with OPS vitrification. The survival rates, maturation rates, fertilization rates, cleavage rates of them were 80. 65% vs 77.50%, 52.00% vs 41.94% , 57.69% vs 61.54%, 60. 00% vs 62.50%, respectively. There was no significant difference in survival rate, maturation rate, fertilization rate, cleavage rate between GV vitrification group and MI vitrification group or between medium HTF group and G - Mops group. There was no significant difference in maturation rate, fertilization rate, cleavage rate of thawed immature oocytes between TCM199 group and IVF -30 group (P 〉 0. 05 ) . Conclusion: The vitrification of human immature ooeytes technology has been initially established.