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人未成熟卵玻璃化冷冻初步实验探讨
  • ISSN号:1001-4411
  • 期刊名称:《中国妇幼保健》
  • 时间:0
  • 分类:R321.1[医药卫生—人体解剖和组织胚胎学;医药卫生—基础医学]
  • 作者机构:[1]福建省妇幼保健院辅助生殖技术研究室,福建福州350001
  • 相关基金:福建省青年科学基金项目[2006-1-14]
中文摘要:

目的:对人未成熟卵母细胞玻璃化冷存保存技术进行初步实验探讨。方法:采用OPS玻璃化冷冻方法,对未成熟的卵子进行冷冻,解冻后体外成熟培养,ICSI受精,观察胚胎培养情况,并对某些影响因素进行探讨。结果:冷冻了GV期卵62个,M I期卵40个。两期卵细胞复苏后存活率、成熟率、受精率和卵裂率分别为:80.65%、77.50%,52.00%、41.94%,57.69%、61.54%,60.00%、62.50%,两组间比较无统计学差异(P〉0.05);采用HTF或G-Mops两种缓冲液配制玻璃化冷冻液冷冻解冻未成熟卵效果无统计学差异(P〉0.05);未成熟卵解冻后置于含性激素的TCM199与单纯IVF30中成熟培养效果无统计学差异(P〉0.05)。结论:应用OPS玻璃化冷冻方法能够有效冻存未成熟卵母细胞。

英文摘要:

Objective: To explore the efficiency of vitrification of hunan immature oocytes. Methods: The human immature oocytes were freezed by OPS vitrification method, then cultured in vitro after thawing, ICSI fecundation, culture conditions were observed and related factors were explored. Results: 62 GV and 40 MI oocytes were freezed with OPS vitrification. The survival rates, maturation rates, fertilization rates, cleavage rates of them were 80. 65% vs 77.50%, 52.00% vs 41.94% , 57.69% vs 61.54%, 60. 00% vs 62.50%, respectively. There was no significant difference in survival rate, maturation rate, fertilization rate, cleavage rate between GV vitrification group and MI vitrification group or between medium HTF group and G - Mops group. There was no significant difference in maturation rate, fertilization rate, cleavage rate of thawed immature oocytes between TCM199 group and IVF -30 group (P 〉 0. 05 ) . Conclusion: The vitrification of human immature ooeytes technology has been initially established.

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期刊信息
  • 《中国妇幼保健》
  • 北大核心期刊(2011版)
  • 主管单位:国家卫生和计划生育委员会
  • 主办单位:中华预防医学会 吉林省医学会
  • 主编:胡国义
  • 地址:长春市建政路971号
  • 邮编:130061
  • 邮箱:zgfybj@sina.com
  • 电话:0431-88929639 88923066
  • 国际标准刊号:ISSN:1001-4411
  • 国内统一刊号:ISSN:22-1127/R
  • 邮发代号:12-94
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版)
  • 被引量:101800