欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的：建立测定比格犬血浆中特拉唑嗪的液相色谱—串联质谱法，研究该药不同剂型的药物动力学特点。方法：取血浆样品0．2mL经液—液萃取后，以甲醇—水—甲酸(70：30：0．5)为流动相，用Diamonsil C18柱分离，采用大气压化学离子源三重四极杆串联质谱，以选择反应监测(SRM)方式进行检测。用于定量分析的离子反应分别为m／z 388→290(特拉唑嗪)和，m/z 256→167(内标，苯海拉明)。结果：特拉唑嗪线性范围为0．2～100．0ng·mL^-1，定量下限为0．2ng·mL^-1，日内、日间精密度(RSD)均小于6．0％，准确度(RE)在±6．4％以内。应用此法研究比较了6只比格犬单剂量口服盐酸特拉唑嗪缓释片及普通片2mg后的药物动力学特点，测得缓释片与普通片的主要药动学参数如下：Tmax(h)分别为4．2±1．0和2．0±1．1，Cmax(ng·mL^-1)分别为44．6±8．1和59．3±13．1，T1／2(h)分别为9．4±1．9和11．0±1．5，AUC0～1(ng·h·mL^-1)分别为624．7±144．1和674．8±194．2。盐酸特拉唑嗪缓释片的Tmax明显大于普通片，而Cmax略小，表明该受试制剂具有缓释特征。结论：首次报道了研究特拉唑嗪犬体内药物动力学特点的方法，该法专属、灵敏、快速，适用于特拉唑嗪制剂的生物等效性研究。
同期刊论文项目
同项目期刊论文
Determination of simvastatin in human plasma by LC/MS/MS: application to the PK study in healthy Chi
Profiling of isovalertatin family aminooligosaccharides extracted from the culture of Streptomyces l
Structural revision of isovalertatin M03, M13, and M23 isolated from the culture of Streptomyces lut
Quantitation of the flavonoid wogonin and its major metabolite glucuronide in rat plasma by LC/MS/MS
Isolation and identification of the glucuronide of 4-(3H-1,2-dihydro-1- pyrrolizinone-2-methylamino)
Sensitive and specific LC/MS/MS assay for dihydroergotamine and its major metabolite in human plasma
Pharmacokinetics and partial thrombo- plastin time after intra-venous recom- binant hirudin variant-
BE study of amlodipine maleate tablet and amlodipine besylate tablet (Norvasc) in healthy Chinese su
PK of a sustained-release formulation of buflomedil after single and multiple doses in healthy Chine
Determination of ketotifen and its conjugated metabolite in human plasma by LC/MS/MS: application to
LC-MS-MS analysis of 2-pyridylacetic acid: application to a pharmacokinetic study in healthy volunte
Anti-tussive activity of benproperine enantiomers on citric acid-induced cough in conscious guinea p
Simultaneous determination of albendazol and its major active metabolite in human plasma using LC/MS
期刊信息
位置：