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中文摘要：
与传统的菌落计数、杂交技术及PCR技术等相比，实时荧光定量PCR技术作为一种检测和定量微生物的方法，具有较高的灵敏度及可重复性，而且反应过程快速，污染较小。作者综述了实时荧光定量PCR技术的定量原理，荧光检测物质，目的基因序列以及其特点，并且列举了在乳酸茵定量检测中的应用，同时对其应用前景进行了展望。
英文摘要：
Real-time fluorescent quantitative PCR （RT-FQ PCR）was a method detecting and quantifying microorganism. Compared with traditional colony count, hybridization and other PCR-technology, RT-FQ PCR has better sensitivity and reproducibility, it was also quicker to perform and has minimized cross contamination. The basic principle of RT-FQ PCR, the chemistries, target gene sequences and characteristic of RT-FQ PCR were described and discussed in this review, furthermore, some applications were presented and the future perspectives were suggested.
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