用91对SSR标记对太湖流域94份粳稻品种进行基因组扫描,分析基因组中等位变异偏分布;调查了水稻幼苗的缺氧反应指数,采用TASSEL软件的GLM方法对标记与幼苗耐缺氧能力进行关联分析;并以携带无效等位基因材料表型均值为对照,鉴别出携带有利等位变异的种质。检测到4个与幼苗耐缺氧能力关联的SSR位点。6个SSR优异等位变异为RM311—176bp、RM317—164bp、RM112—127bp、RM20—205bp、RM317—157bp和RM311—170bp,携带上述优异等位变异的品种为镇稻88、白芒糯、开青、有芒早稻、粗秆黄稻和C堡。
The purpose of this study were to detect elite alleles associated with anaerobic tolerance of seedling from Oryza sativa japonica varieties growing in Taihu Lake region, and to screen typical carrier genotypes of these alleles. The genotyping data of 91 simple sequence repeat (SSR) markers on representative samples of 94 varieties were used in the present study, and the anaerobic responsive index of seedling were investigated. The association a- nalysis between SSR loci and the anaerobic tolerance was performed using General Linear Model program in Tassel 2.0. Elite alleles and their carrier materials were determined on the basis of the result of comparisons between the average phenotypic value of accessions with the specific allele and that of accessions with "null allele". Four SSR loci associated with seedling anaerobic tolerance were detected. Six elite alleles were RM311-176bp, RM317- 164bp, RMl12-127bp, RM20-205bp, RM317-157bp and RM311-170bp. Zhendao88, Baimangnuo, Kaiqing Youmangzaodao, Cuganhuangdao and C-bao were carrier genotypes having the elite alleles for seedling anaerobic tolerance.