[目的]定位粳稻农艺性状对外源赤霉素敏感性的QTL,为选育和改良高敏感性不育系提供理论依据。[方法]以粳稻秀水79与C堡及其杂交后代衍生的重组自交系群体260个株系为研究材料,研究其4个农艺性状对外源赤霉素处理的敏感性并对其QTL进行定位。敏感性用反应指数衡量,反应指数越大,敏感性越高。采用WinQTLCartographer2.5软件的复合区间作图法(CIM),在南京农业大学作物遗传与种质创新国家重点实验室构建的粳稻SSR分子标记连锁图谱的17个连锁群上每隔2cm计算LOD值。采用排列组合1000次方法确定LOD阈值,以保证全基因组检测到的QTL犯Ⅰ类错误概率小于5%。当实际求得的LOD值大于LOD阈值时,就认为该区段存在1个QTL,其置信区间为LOD峰值向下1个LOD值单位区间。QTL命名遵循McCouch等规则。估算每个QTL的贡献率和加性效应。[结果]控制剑叶角度对赤霉素敏感性的QTL共有3个,分别位于第3、9、9染色体上,其贡献率分别为5.59%,13.00%和11.80%,增效等位基因分别来自秀水79,C堡和C堡;控制株高对赤霉素敏感性的QTL共有2个,分别位于第1和8染色体上,其贡献率分别为8....
[Objective] The research aimed to map QTL for the sensitivity of the traits related outcrosssing of Japonica rice to exogenous GA3 and provide theoretical basis for breeding and improving the high-sensitivity sterile line.[Method] Taking Japonica rice Xiushui 79 and C bao and their recombinant inbred line population 260 lines as test materials,the sensitivity of 4 traits related outcrossing to Exogenous GA3 and their QTL mapping were studied by using composite interval mapping..[Result]Three QTLs,which cont...