中文摘要：

目的 研究窄谱中波紫外线（NB-UVB）对培养的正常人角质形成细胞（KC）增殖和肝素结合表皮生长因子样生长因子（HB-EGF）mRNA表达的影响。方法 用50mJ／cm^2和100mJ／cm^2 NB-UVB照射正常人KC后继续培养12h，用MTT法和实时荧光定量RT—PCR法分别检测KC增殖和HB-EGF mRNA的改变。结果 NB-UVB照射正常人KC后，可剂量依赖抑制KC的增殖和上调HB-EGF mRNA的表达。50M／cm^2和100mJ／cm^2 NB-UVB照射后，分别使KC增殖抑制5．4％和8．7％，使HB-EGF mRNA增加到正常对照组的1．7倍和2．5倍。结论 NB-UVB抑制KC的增殖部分是通过上调HB-EGF的表达介导的。

英文摘要：

Objective To investigate the alteration of cell proliferation and heparin-binding epidermalgrowth-factor-like growth factor （HB-EGF）mRNA expression in cultured normal human keratinocytes after NB-UVB irradiation. Methods After irradiation with 50 mJ/cm^2 and 100 mJ/cm^2 NB-UVB in normal human keratinocytes, the proliferation potency of the cells was detected by MTT colorimetric assay and the expression of HB-EGF mRNA was examined by real-time quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results Both keratinocytes growth inhibition and HB-EGF mRNA expression were dose-dependently induced by NB-UVB. The proliferation of keratinocytes was inhibited by 5.4% and 8.7%, and the expression of HB-EGF mRNA was elevated by 1.7- and 2.5-fold after irradiation with 50 mJ/cm^2 and 100 mJ/cm^2 NB-UVB, respectively. Conclusion The growth inhibition induced by NB-UVB in normal human keratinocytes may be mediated at least in part by upregulated HB-EGF expression.