中文摘要：

研究了利用Affymetrix的欧洲葡萄基因表达芯片检测中国野生葡萄基因表达的可行性，并检测了葡萄白粉病菌（Uncinular necator）人工接种诱导48h后的基因表达情况。结果表明：该芯片可以在感病材料中检测出表达的基因11906个，抗病材料中检测到表达的基因11839个，在二者中同时检出表达的基因11839个，能够检出的基因数占该芯片基因探针组总数的71．3％，因此用欧洲葡萄基因芯片检测中国野生葡萄中基因表达水平是可行的；利用此芯片检测出在人工接种诱导后，抗病材料中和感病材料相比表达上调的基因共1920个，占检测出基因总数的16．21％；表达下调的基因数1760个，占检测出基因总数的14．87％；表达上调和下调的幅度（signal log ratio）都在1—7倍之间。

英文摘要：

The feasibility of using Affymetrix Vitis vinifera gene expression microarray to detect the gene expression of Chinese wild grape was studied, and the gene expression after 48 h artificial inoculation with Uncinular necator was detected. The results showed that the microarray can detect the expression of 11 906 genes in susceptible material （S）, while 11 839 genes in resistant individual （R） and 11 839 genes in both R and S. The detected genes accounted for 71.3% of the total 16 602 probe groups. That means Affymetrix V. vinifera microarray can be efficiently used for gene expression analysis of Chinese wild grapes. Compared to the S plant, 1 920 up-regulated and 1 760 down-regulated genes were detected in R plant, accounting for 16.21% and 14. 87% of total detected genes respectively, up-and down-regulated rate （signal log ratio） are among 1 to 7.