中文摘要：

依据中国野生葡萄（Vitis pseudoreticulata）抗黑痘病（Sphaceloma ampelinum）基因RAPD标记OPS03-1354的全长序列,设计合成了两个寡聚核苷酸R1（5＇-CAGAGGTCCCTAGCTAAT-3）＇和R2（5＇-ACA ATCACCCAACTCCTC-3）＇作为引物。首先对获得该RAPD标记的种间杂交组合白河-35-1（V.pseudoret-iculata clone Baihe-35-1）×佳利酿（V.vinifera cv.Carignane）亲本及其F251株进行PCR分析,其中寡聚核苷酸R2能在抗黑痘病植株中扩增出约1100bp的DNA片段。进一步用R2对另一种间杂交组合广西-1（V.pseudoreticulata clone Guangxi-1）×京可晶（V.vinifera cv.Jingkejing）F1339株进行PCR检测,R2能在抗病植株中扩增出约1100bp的DNA片段。最后用R2对48份葡萄资源包括中国野生葡萄、美国野生葡萄和欧洲葡萄进行了PCR检测,在抗病株系和品种中也能扩增出这一片段。结果表明,获得的约1100bp特异片段为葡萄抗黑痘病基因的序列特异性扩增区（SCAR）标记,凡扩增出该SCAR标记的植株即是抗黑痘病基因的携带者和抗黑痘病性状的表达者。将该SCAR标记克隆与测序,其实际长度为1110bp,命名为SCS03-1110。获得该SCAR标记的18bp寡聚核苷酸R2（5＇-ACAATCACCCAACTCCTC-3）＇具有检测葡萄抗黑痘病基因的DNA探针作用。

英文摘要：

Based on the sequence of RAPD marker OPS03-1354 linked to anthracnose（Sphaceloma ampelinum） resistance gene,two oligomers,R1（5＇-CAGAGGTCCCTAGCTAAT-3＇） and R2（5＇-ACAATCACCCAACTC CTC-3＇）,were designed and synthesized,and used as special primer for PCR analysis among the parents and 51 F1 individuals from interspecific cross combination Vitis pseudoreticulata Baihe-35-1×V.vinifera Carignane.A DNA band about 1 100 bp could be amplified among resistant plants using primerR2,and was present in resistant plants of 339 F1 individuals from interspecific cross combination Guangxi-1（V.pseudoreticulata）×Jingkejing（V.vinifera） and of 47 lines（varieties） of grape resources,including Chinese wild Vitis,American wild Vitis and European grape,by PCR analysis.The result showed that the DNA band about 1 100 bp was a sequence-characterized amplified region（SCAR） markerlinked to anthracnose-resistance gene in grape.The plants with the SCAR marker were the carrierand expression of anthracnose-resistance gene.Cloning and sequencing of the SCAR marker were carried out.The length of the SCAR marker was actually 1 110 bp and it was named SCS03-1110.This research reveals that the oligomer R2（5＇-ACAATCACCCAACTCCTC-3＇） as a DNA probe can be used to identify anthracnose-resistance gene of grape germplasm resources and hybrids from anthracnose-resistance breeding program by PCR assay.