中文摘要：

目的：探索针刺抗哮喘大鼠血清差异组份的研究方法。方法：用蛋白质SDS-PAGE技术，对针刺抗哮喘血清进行组份差异分析。结果：SDS—PAGE电泳显示了针刺与不针刺血清中差异蛋白的存在：1）与正常对照组相比，ADXAcup组血清组份Ⅰ和Asthma_Acup组血清组份Ⅰ，均有一染色增高条带，其分子量为62．1kDa；2）与正常对照组相比，ADXAcup组血清组份Ⅲ有二条染色增高条带，其分子量分别为36．1kDa、12．3kDa；3）与正常对照组相比，ADXAcup组血清组份Ⅲ有一条染色降低条带，其分子量为34．6kDa。提示针刺对血清蛋白具有上调和下调的双向调节作用。结论：针刺抗哮喘大鼠血清中存在不同的差异组份。针刺对血清蛋白具有上调和下调的双向调节作用。

英文摘要：

Objective： To explore a method of analyzing the components of anti-asthma activity in serum derived from rats treated with acupuncture. Methods： SDS-PAGE was used to analyze the diversity of the components of serum derived from rats treated with or without acupuncture treatment. Results： SDS-PAGE images displayed that there are one deeply dyed band, whose molecular weight is 62.1 kDa, in the component I from asthma acupuncture group and ADX-asthma acupuncture group, two deeply dyed bands, whose molecular weight are 36. lkDa and 12.3 kDa, respectively, in the component Ⅲ of ADX-asthma acupuncture group, and one dyed band, whose molecular weight is 34.6 kDa, in the segment Ⅲ of ADX-asthma acupuncture group. Conclusion： Acupuncture treatment could produce different components in the serum of rats, and may have bidirectional regulation on protein components in serum.