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中文摘要：
目的探讨缓激肽持续作用引起的胶质瘤细胞内钙离子浓度变化（[Ca^2＋]i）及机制。方法培养大鼠C6胶质瘤细胞，采用[Ca^2＋]i测定和免疫荧光细胞化学鉴定的方法，观察持续给予缓激肽后胶质瘤细胞内[Ca^2＋]i的变化和缓激肽B2受体在细胞内的分布。结果在第一次给予缓激肽时，引起[Ca^2＋]i的峰值最大，随着给予缓激肽次数的增加，缓激肽诱导的[Ca^2＋]i荧光强度峰值逐渐下降，在第5次加入缓激肽时，[Ca^2＋]i荧光强度峰值已无显著变化。在此过程中，缓激肽B1受体发生内化，并与胞浆中的质膜微囊蛋白caveolin-1结合。结论持续给予缓激肽可引起胶质瘤细胞内[Ca^2＋]i浓度规律变化，caveolin-1可能参与此过程。
英文摘要：
Objective To study the [Ca^2＋]i change and mechanism induced by continuous stimulation of bradykinin in glioma cells. Methods [Ca^2＋]i assay and immunofluorescence staining were used to observe the [Ca^2＋]i change and the location of bradykinin B2 receptors after the continuous treatment with bradykinin in cultured rat C6 glioma cells. Results With the first stimulation of bradykinin, [Ca^2＋]i fluorescence intensity ascends to peak and descends gradually as the continuous stimulation of bradykinin, and with no significant change of [Ca^2＋]i fluorescence intensity at the fifth stimulation. During this process, combination of bradykinin B2 receptors with caveolin-1 was observed in cytoplasma. Conclusions Continuous administration of bradykinin can cause the regular change of [Ca^2＋]i concentration in glioma, in which caveolin-1 may be involved.
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