中文摘要：

目的：筛选出增生型胸腺组织中的重症肌无力（MG）相关蛋白，并对其作出鉴定。方法：利用已经建立的胸腺组织蛋白质组双向电泳技术获得分离良好的SDS—PAGE凝胶，将凝胶中的蛋白点电泳转移到硝酸纤维素膜上，分别以异体MG患者血清、正常人血清和自身免疫病患者血清为第一抗体，进行免疫印迹试验；然后，在参考凝胶上挖取MG组特异显色点所对应的蛋白质点，经胶内胰蛋白酶消化，对所得多肽混合物进行基质辅助激光解析电离飞行时问质谱分析，得到肽质量指纹图谱（PMF）数据，登录网站http：／／www．matrixscience．com，用Mascot程序检索NCBInr数据库，鉴定蛋白质。结果：免疫印迹试验共获得5个MG特异显色点，编号为2、4、6、7、9；经质谱分析得到5个蛋白质PMF图谱，经数据库检索鉴定，这些蛋白分别为：异肽酶T、核因子p97、亚铁血红素结合蛋白2、肿瘤翻译受控蛋白1和角蛋白10。结论：利用免疫印迹试验筛选和质谱分析，成功鉴定出增生型胸腺组织中的5个MG相关蛋白，为进一步探讨这些蛋白在MG发生发展中的作用奠定了基础。

英文摘要：

Aim ：To screen and identify the myasthenia gravis（MG） related proteins from MG hyperplastic thymus tissue, MethodS：Compared with the control （normal serum） and AID （autoimmune disease serum） , MG serums were incubated with protein spots on the NCM. After western blotting, the colored corresponding protein spots in 2-DE gel were cut and done in-gel digestion, and the mixture of peptides was send to MALDI-TOF-MS. Protein identification was finished by searching the PMF（peptide mass fingerprinting） data in NCBInr protein data bank using Mascot program at http：//www. matrixscience, com. Results ： 5 MG specificity colored protein spots were obtained and marked NO. 2, NO, 4, NO. 6, NO. 7, and NO. 9,which were considered as isopeptidase T, nuclear factor p97, heme binding protein 2, translationally-controlled 1, and keratin-10, respectively, by searching the NCBInr protein data bank after inputting the PMF data, Conclusion ： Utilizing 2-DE techniques and western blotting assay, 5 MG related proteins are obtained and successfully identified,