中文摘要：

目的探讨核胞浆转运蛋白α-55（karyopherin alpha-5，KPNA5）在重症肌无力（myasthenia gravis，MG）患者胸腺组织中的表达情况。方法分别利用荧光定量聚合酶链反应（fluorescence quantitative polymerase chain reaction，FQ—PCR）和免疫组化染色方法，检测MG组20例患者与对照组10名受试者胸腺组织中KPNA5 mRNA和蛋白分子的表达。结果MG组和对照组胸腺组织中，KPNA 5 mRNA的表达水平分别为（0．71±0．08）和（0．57±0．02），两者之间的差异有统计学意义（P〈0．05）；KPNA 5蛋白阳性细胞均数分别为（8．99±0．49）和（5．88±0．69），差异有统计学意义（P〈0．05）。结论KPNA5分子在MG胸腺组织中表达增高，可能参与了MG的发生，是MG相关基因。

英文摘要：

Objective To study the expression of KPNA 5 in the thymus tissues of the patients with myasthenia gravis. Methods The expressions of KPNA 5 mRNA and protein in the thymus tissue of MG and the normal control were detected using fluorescent quantitative Polymerase Chain Reaction （FQ-PCR） and immunohistochemical SP method. Resuits The expression of KPNA 5 gene at mRNA level in the thymus tissues of MG （ n = 20,0. 71±0. 08 ） was significantly higher than that of the control group （ n = 10,0. 57±0. 02） （ P 〈 0. 05 ）. The number of KPNA 5 positive cells in the thymus tissues of MG （ n = 18,8.99±0. 49） was remarkably higher than that of the control group （ n = 10,5.88±0. 69） （ P 〈 0. 05 ）. Conclusions There is high expression of KPNA 5 in the tissue of MG patients which may be associated with the generation and development of MG.