中文摘要：

目的建立分辨率高和重复性好的重症肌无力（MG）增生型胸腺组织11KD差异（异常）蛋白带的二维凝胶电泳图谱。方法首先采用SDS-PAGE电泳筛选出含有11KD差异（异常）蛋白条带的MG增生型胸腺组织,然后富集11KD蛋白条带。以17cm pH3-10固相pH梯度胶条（immobilized pH gradient,IPG）做第一向等电聚焦（IEF）,12%SDS聚丙烯酰胺凝胶为第二向进行双向电泳（2-DE）,PD Quest 7.1软件分析电泳图谱。结果11KD差异（异常）蛋白条带的出现率为72.2%;通过双向电泳,建立分辨率高和重复性好的重症肌无力（MG）增生型胸腺组织11KD差异蛋白带的双向凝胶电泳图谱,进一步分离11KD差异（异常）蛋白带,共得到（30±6）个蛋白点。结论通过蛋白质组技术快速建立重症肌无力11KD差异（异常）蛋白表达图谱,为进一步研究差异表达蛋白的功能提供实验基础。

英文摘要：

Objective To establish two-dimensional gel electrophoresis （2-DE） profiles of hyperplastie thymus with 11KD strip of myasthenia gravis patients. Methods The thymus tissue containing 11KD differential or abnormal strap was bolted with SDS-polyaerylamide gel eleetrophoresis. 11KD protein strips were collected and cleaned. The 2-DE system with the first dimension using isoeleetrofoeusing eleetrophoresis with 17cm pH3-10 IPG gel and the second dimension using 12 % SDS-poly-aerylamide gel eleetrophoresis were employed to obtain the 2-DE maps, and analyzed by PDQuest gel image software. Results 72.2% hyperplastie thymus tissues contained 11KD protein strip. The two-dimensional polyaerylamide gel eleetrophoresis profiles of 11KD proteins were successfully established by 2-DE and obtained （30±6） protein spots. Conclusion The 2-DE profiles of 11KD proteins were successfully established and can be an experimental basis for further research of myasthenia gravis.