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中文摘要：
目的：建立栀子HPLC数字化指纹图谱并进行统一化研究。方法：采用反相高效液相色谱法，使用CenturySILC。。BDS柱（20cm×4．6mm，5μm），以1％醋酸水一1％醋酸乙腈为流动相，低压线性梯度洗脱，检测波长265nm，柱温（30．0±0．15）℃，进样量5μL，测定10批不同产地栀子HPLC指纹图谱。以色谱指纹图谱指数F和色谱指纹图谱信息量指数，等指标定性、定量评价不同产地栀子药材质量。用方向余弦阐明大峰对相似度的影响，建立了比率定性相似度的消除方法。结果：以栀子苷峰为参照物峰，确定35个共有峰，建立了栀子HPLC数字化指纹图谱和统一化指纹图谱。结论：所建立的指纹图谱具有较好的精密度和重现性，可作为栀子药材质量控制和真伪鉴别的依据。
英文摘要：
AIM ： To establish a novel method for the overall quality control of Fructus Gardeniae by HPLC digi- tized fingerprint and its normalization, METHODS： The chromatographic fingerprints were obtained by injecting 5 μL of the sample solution each time on a Century SIL C18 BDS column （20 cm×4.6 mm, 5 μm） with the gradient elution solvent system composed of 1% acetate acid-water and 1% acetate acid- acetonitrile. The flow rate was 1.0 mL/min, the column temperature was maintained at （30±0.15 ）℃ and the detection wavelength was set at 265 nm. The chromatographic fingerprints were evaluated by the digitized parameters, such as the chromatographic fingerprint index （F） , quantitative similarity, etc,. The influence of big peak on similarity was elaborated by di- rection cosines, and its cancellation was achieved by the qualitative similarity of peak area ratio. RESULTS： 35 co-possessing peaks were selected as the fingerprint peaks of Fructus Gardeniae by taking Geniposide peak as the reference peak. The HPLC digitized fingerprint and its normalization were implemented. CONCLUSION： This method with good precision and reproducibility can be useful in the quality control of Fructus Gardeniae.
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Qualitative and quantitative assessment of the HPLC fingerprints of Ginkgo biloba extract by the inv
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