目的:建立刺五加HPLC数字化指纹图谱。方法:采用反相高效液相色谱法,使用Century SIL BDS(20 cm×4.6mm,5μm)柱,以1%醋酸水-1%醋酸乙腈为流动相,线性梯度洗脱,流速1.0 mL/mL,检测波长265 nm,柱温(30.0±0.15)℃,进样量10μL。以定性、定量相似度和色谱指纹图谱指数等数字化指标评价药材质量。结果:以绿原酸峰为参照物峰,确定31个共有指纹峰,建立了刺五加HPLC数字化指纹图谱。应用色谱指纹图谱指数F和相对指数Fr参数对不同产地刺五加HPLC指纹图谱的超信息特征进行了数字化评价。结论:所建立的HPLC指纹图谱具有较好的精密度和重现性,适用于刺五加药材的质量控制。
AIM : To establish a novel method for the overall quality control of Radix et rhizoma seu caulis Acanthopanacis senticosi on the ground of HPLC digitized fingerprint. METHODS: The chromatographic fingerprints were obtained by injecting 10 μL of the sample solution each time on a Century SIL BDS column(20 cm ×4.6 mm, 5 μm) with the gradient elution solvent system composed of 1% acetate acid water and 1% acetate acid acetonitrile. The flow rate was 1 mL/min, the colunm temperature was maintained at (30± 0. 15 )℃ and the detection wavelength was set at 265 nm. RESULTS : 31 co-possessing peaks were selected as the fingerprint peaks of Radix et rhizoma seu caulis Acanthopanacis senticosi and the similarities between the chromatographic fingerprints and the herbal drugs were calculated taking chlorogenic acid peak as the reference peak. The chromatographic fingerprints also were evaluated by the chromatographic fingerprint index ( F), the relative index (Fr). CONCLUSION: This method with good precision and reproducibility can be useful for the quality control of Radix et rhizoma seu caulis Acanthopanacis senticosi.