中文摘要：

人胚胎干细胞（human embryonic stem cell,hESCs）是早期胚胎或原始性腺中分离出来的一类细胞,它具有无限增殖、自我更新和全能分化的特性。无论在体内还是体外环境,人胚胎干细胞都能分化为机体几乎所有类型的细胞。基于其全能分化性,胚胎干细胞成为治疗各种退行性疾病的理想细胞来源。然而,在目前培养条件下所建立的胚胎干细胞株,仍然存在动物源性物质潜在污染的问题。因此,更优化的建株及培养条件十分重要。

英文摘要：

Human embryonic stem cells（hESC） are from early embryos and primary gonads, they have the features of infinite proliferation, self-renewal and totipotency. They can differentiate into all type of somatic cells in vitro and in vivo. Based on its totipotency, hESC has become an important source cells in treating degenerating diseases. However, the hESC lines generated under current generating and culturing systems could not avoid the potential contamination of animal-derived substances. It is critical to formulate an optimal generating and culturing system for hESC.