中文摘要：

目的：探讨不同浓度的芬太尼对食管鳞癌细胞株Eca109增殖和凋亡的影响。方法：选用不同浓度的芬太尼（0.5、5、50、500ng/ml）干预细胞。采用四甲基偶氮唑蓝（MMT）法检测细胞增殖,流式细胞仪Annexin V/PI双染色法测定细胞凋亡。结果：各组芬太尼孵育Eca109细胞24h后,对细胞的增殖无明显影响;孵育48h后,与对照组相比,各浓度组均能显著抑制细胞的增殖（P〈0.05）,且呈浓度和时间依赖性。芬太尼孵育Eca109细胞48h,与对照组相比,各浓度组均能诱导早期凋亡和晚期凋亡,呈浓度依赖性（P〈0.05）。结论：芬太尼可抑制食管鳞癌细胞株Eca109的增殖并诱导凋亡。

英文摘要：

Objective： To investigate the effects of fentanyl on proliferation and apoptosis in esophageal squamous cell carcinoma cell line Eca109. Methods： Eca109 cells cultured to logarithmic phase were randomly treated with different dose of fentanyl（ 0. 5,5,50,500 ng/ml）. MTT was applied to test the effect on cell proliferation and Annexin-V/PI assay was used to study its effect on apoptosis. Results： MTT test revealed that fentanly had no effect on proliferation at 24 h,but inhibited cell proliferation at 48 h and 72 h in a dose and time department manner. Annexin-V/PI assay demonstrated that fentanly induced more early and late apoptosis in Eca109 cells with increased drug dose.Conclusion： Fentanyl inhibited cell proliferation and induced apoptosis in ESCC cell line Eca109.