中文摘要：

目的：探讨YAP及磷酸化YAP在胃腺癌组织中的表达及其与胃腺癌发生发展的关系。方法：在60例胃腺癌患者中,应用免疫组化检测YAP及磷酸化的YAP蛋白在胃腺癌组织、癌旁正常组织中的表达情况。利用siRNA抑制细胞中YAP蛋白的表达,MTT检测胃腺癌细胞的增殖能力。结果：YAP蛋白可同时表达于细胞浆及细胞核中,而磷酸化的YAP主要表达于细胞浆中。与正常胃组织相比,胃腺癌组织中YAP蛋白的表达明显升高（P〈0.05）,而磷酸化的YAP却显著减少（P〈0.05）。胃腺癌YAP的表达与患者性别、年龄、肿瘤的分级及分期无相关性,而与胃癌瘤体大小相关（P〈0.05）。利用siRNA抑制YAP表达可显著降低胃腺癌细胞的增殖能力。结论：胃腺癌中YAP的高表达及磷酸化YAP的低表达可能参与了胃癌的发生发展。

英文摘要：

Objective：To explore the expressions and significance of Yes -asociated protein（YAP） and phospho- rylated YAP in gastric adenocarcinoma. Methods：Human gastric adenocarcinoma tissues and pericarcinomatousc nor- mal gastric tissues were collected and the protein expression of YAP and phosphorylated YAP（pYAP） were evaluated using immunohistochemistry. Then, human gastric cancer SGC -7901 cells were transfected with YAP- siRNA（ shR- NA）. The expression levels of YAP were detected by Western Blots. Effects of siRNA - mediated knockdown of YAPon cell proliferation were assessed by MTF. Results： The expression of YAP was observed in both cytoplasm and nu- cleus ,while pYAP was observed only in cytoplasm. The expression of YAP was higher in gastric adenocarcinoma than in the normal tissues （P 〈 0.05）. However, pYAP in gastric adenocarcinoma was lower（P 〈 0.05 ）. The YAP overex- pression was associated with tumor size, but not other clinical characteristics. Moreover, knockdown of YAP could in- hibit the proliferation of gastric cancer cells. Conclusion ： YAP and phosphorylated YAP may play a key role in the in- itiation and progression of zastric adenocarcinoma.