中文摘要：

目的对针刺抗哮喘差异表达蛋白的生物学功能和分子网络进行分析,探讨生物信息学方法在针灸研究中的应用和思路。方法利用分类系统（PANTHER）、信号通路交互作用数据库（Pathway Interaction Database,PID）、分子网络作用软件（Ingenuity Pathway Analysis,IPA）分别对针刺抗哮喘差异表达蛋白进行分子生物功能注释、信号通路、蛋白相互作用和分子调控网络分析。结果多个针刺抗哮喘差异表达蛋白与免疫系统功能密切相关,涉及了RhoA信号通路、Toll样受体信号通路、嗜酸性粒细胞Ccr3信号通路、T细胞IL-2R beta活化通路等生物通路,通过调控下游细胞因子,影响CD4分子的功能,发挥免疫调节作用。结论通过对针刺抗哮喘差异表达蛋白的生物信息学分析获得了针刺抗哮喘作用免疫相关分子通路和调控靶点,为进一步研究针刺抗哮喘的免疫相关分子机制奠定基础。

英文摘要：

Objective To analyze the biological functions and molecular networks of differentially expressed proteins in anti-asthma acupuncture and explore the application of bioinformatic methods to and ideas for acupuncture research. Methods PANTHER classification system, Pathway Interaction Database （PID） and Ingenuity Pathway Analysis （IPA） software were used for molecular biological function annotation of differentially expressed proteins in anti-asthma acupuncture and analyses of signaling pathways, protein-protein interactions and molecular regulatory networks. Results Several differentially expressed proteins in anti-asthma acupuncture were closely related to immune system function and involved in biological pathways including RhoA signaling pathway, Toll-like receptor signaling pathway, eosinophils Ccr3 signaling pathway and T cell IL-2R beta activation pathway. They produced an immunoregulatory effect by modulating downstream cytokines and influencing CD4 molecule function）. Conclusion Immune-related molecular pathways and regulation targets involved in the anti-asthma effect of acupuncture are obtained by bioinformatic analysis of differentially expressed proteins in anti-asthma acupuncture, which provides a basis for further research on the immune-related molecular mechanisms of acupuncture treatment for asthma.