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中文摘要：
目的：建立一种定量测定大鼠淋巴细胞培养上清液中总IgG（免疫球蛋白G）含量的双抗体夹心ELISA（酶联免疫吸附试验）检测方法。方法：用方阵实验确定包被抗体、检测抗体的最佳工作浓度；绘制标准曲线，确定线性范围；评价标准曲线的可重复性、精密度和可应用性。结果：包被抗体和检测抗体的最佳效价分别为2ug／ml和1：4000稀释；检测的线性范围为0．25—16ng／ml。经方法学评价，可重复性和精密度较高，应用性较强。结论：该方法灵敏度高，重复性好，可作为科研过程中检测大鼠淋巴细胞培养上清液总IgG含量的一种精确、方便、可靠的方法。
英文摘要：
Objective： To establish a quantitative double-antibody sandwich ELISA detecting method for total IgG content of cultured rat-lymphocyte supematant. Methods： Detect the optimal concentrations of coated antibody and enzyme-antibody with a matrix experiment. Construct a calibration curve and determine the linear rang. Evaluation repeatability, precision and applicability of the calibration curve. Results： The optimal concentration of coated antibody is 2 ug/ml and the optimal dilution ratio of enzyme-antibody is 1：4000. The linear rang of calibration curve is 0.25-16 ng/mL. The repeatability, precision and applicability are excellent. Conclusion： This ELISA detecting method is convenient and reliable to detect total IgG content of cultured rat-lymphocyte supernatant.
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