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中文摘要：
在相同工艺条件下对大肠杆菌菌悬液作超临界CO2处理,对不同时间段萃取液的成分进行分析。结果表明,萃取物主要有两大类：非极性脂肪酸类及极性较弱的小分子氨基酸类物质,均为细胞的功能性物质。证实在超临界CO2杀菌过程中确实发生了萃取作用。
英文摘要：
Functional components in cytoplasm,cell membrane and cell wall can be dissolved by supercritical carbon dioxide.As a result,the cells will be damaged and die due to inactivation.The extraction mechanism during supercritical carbon dioxide sterilization was explored in this study.An Escherichia coli suspension at a concentration of 108 CFU/mL was prepared and treated with supercritical carbon dioxide and composition analysis was performed on the extracts obtained at different time points.The results indicated that both non-polar fatty acids and polar weak small amino acids were the functional components in cells.This investigation demonstrates that extraction indeed occurs during supercritical carbon dioxide sterilization.
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