通过检测大肠杆菌体内H^+-ATP酶的活性、利用氨基酸自动分析仪测定萃取物的氨基酸成分、扫描电镜对处理前后的大肠杆菌进行观察,比较考察高压CO2对大肠杆菌菌粉和菌悬液的杀菌效果。结果表明:高压CO2杀灭大肠杆菌不是由某一机理单独作用的,而是由机械破裂致死机制、萃取机制和酸化机制共同作用的结果。
The efficacies of high pressure carbon dioxide (HPCD) treatment for inactivating dried culture powder and suspension of E. coli were comparatively evaluated by measuring the activity of H+-ATPase in E, coli, determining the amino acid composition of HECD extracts using an amino acid analyzer, and observing the morphology of E. coli cells under a scanning electron microscope (SEM) before and after HPCD treatment. The results obtained demonstrated that the inactivation of E. coli by HPCD was achieved jointly by mechanical rupture, extraction and acidification rather than a single action.